mRNA Therapeutics and Molecular Biology

Location: Biological E Ltd, Vaccines, R&D

Education: Masters degree in a relevant field with 6-8 years of industry experience (or) Ph.D. with 0-3 yrs experience

Position Summary

The selected candidate will be part of the mRNA Therapeutics team and is expected to bring strong expertise in molecular biology with hands-on experience in mRNA and cell & gene therapy workflows. The role requires a detail-oriented professional capable of executing experimental work, analyzing results, and maintaining high laboratory and documentation standards.

Key Responsibilities

• Perform molecular biology and proteomics techniques including agarose gel electrophoresis, SDS-PAGE, PCR, RT-PCR, qPCR, Western blotting, ELISA, DNA/RNA purification, gene assembly, cloning, and nucleic acid/protein quantification
• Execute plasmid scale-up (maxi and mega scale), in vitro transcription (IVT) reactions, preparation of denaturing gels, and mRNA purification and quantification
• Maintain mammalian cell lines, perform cell culture, transfections, and analyze protein expression through lysis and downstream assays
• Experience in Nucleic acid research with exposure to different mRNA modalities like linear, circular and self amplifying mRNA
• Conduct nucleic acid extraction from diverse sources including bacteria, viruses, blood, animal tissues, cell lines, and FFPE/frozen tissues
• Design primers, perform cloning and conserved sequence analysis, and develop SNP-based genotyping assays and allelic discrimination using qPCR and digital PCR platforms
• Prepare, store, and maintain microbial and recombinant stock cultures
• Adhere to good laboratory practices (GLP) and maintain accurate, comprehensive documentation
• Analyze and interpret experimental data using tools such as JMP/Design Expert. GraphPad Prism and MS Excel
• Ensure proper organization of laboratory workflows, materials, and records
• Collaborate effectively within the team and contribute to a productive research environment
• Perform basic molecular biology and proteomic techniques like nucleic acid isolation and purification, PCR-based amplification (RT-PCR, qPCR), in vitro transcription (IVT), RNA handling and analysis and protein expression analysis.
• Prepare libraries for sequencing applications, including mRNA, microRNA, small RNA, DNA, transcriptome, exome, whole genome, and Nanopore sequencing
• Conduct nucleic acid extraction from diverse sample types, including bacteria, viruses, blood, animal tissues, cell lines, and FFPE/frozen tissues
• Design primers and perform cloning, conserved sequence analysis, and SNP-based genotyping assays, including allelic discrimination using qPCR and digital PCR platforms
• Has to have hands-on experience in NGS data analysis, interpretation and Nanopore workflows.
• Analyze and interpret experimental data using tools such as JMP/Design expert, Geneious, GraphPad Prism and MS Excel.
• Prepare detailed technical reports and scientific documentation, ensuring data is presented in a clear, structured, and comprehensive manner to support interpretation, decision-making, and regulatory requirements.
• Maintain accurate and up-to-date laboratory records, including experimental procedures, observations, and results, in compliance with good documentation practices (GLP).
• Ensure strict adherence to documentation standards, including data integrity while supporting a well-organized and compliant laboratory environment